Thrombosis kit

REF 71-01

(Instructions for use)

Diagnostic kit for detection of hereditary thrombophilia.

Principle of method:

Assay principle is based on simultaneous detection of polymorphisms in the F5, F2 and MTHFR genes by means of multiplex PCR with subsequent restriction and polyacrylamide or agarose gel electrophoresis.

Simultaneous detection of 3 SNPs:

• 1691G/A (rs6025) SNP in F5 gene
• 20210G/A rs1799963) SNP in F2 gene
• 677C/T (rs1801133) SNP in MTHFR gene.

Investigated material: DNA sample

Method working range: single nucleotide polymorphism (SNP)

Composition of diagnostic kit:

• PCR mixes
• Positive control for PCR and restriction
• DNA-polymerase
• Restriction enzymes
• Buffers for restriction enzymes.

Kit characteristics

General procedure:

1. PCR
2. Enzymatic cleavage of PCR products
3. Polyacrylamide or agarose gel electrophoresis and the subsequent visualization in passing UV.

Number of clinical samples: 40

Time of carrying out of diagnostics:

• min — 6 hours
• max — 24 hours

Storage conditions: -18 °Ñ bis -22 °Ñ

Shelf life: 12 months

The necessary equipment: PCR-box, thermocycler, minivortex, centrifuge, thermostat, set of pipettes with variable volume, standard equipment for polyacrylamide or agarose gel electrophoresis.